Abstract：Long lifespan and late maturation make it difficult to establish gamete maturity and breeding age of captive endangered Chinese sturgeon, Acipenser sinensis. This greatly handicaps timely breeding and future conservation stocking efforts. We used ultrasound imagery and sex steroids to determine the gender and gonadal maturity stage of captive Chinese sturgeon (age, 10-17 years old). The echogenicity of the reproductive organs and the respective morphology of the gonads were described and two quantitative parameters p(o) (proportion of the ovary to the entire reproductive organs) and d (thickness of the reproductive organs) were measured to characterize sex and maturity stage of Chinese sturgeon. Females were accordingly placed fish into several categories: F-II (F-II(-), F-II, F-II(+)), F-III (F-III, F-III(+)) and F-IV (F-IV, F-IV(+)) and F-VI, and males as M-II, M-III, M-IV, M-V and M-VI,. The accuracy of gender and maturity stage determination provided by ultrasonographic methods was 72.7% for F-II(-) ovary (n = 11) and 76.2% for MII testis (n = 42). Accuracy of sex and maturity determination using only serum sex steroid of testosterone (T) and estradiol-17 beta (E-2) was low (58-73%, depending on maturity stage). However, when the two methods were used together, accuracy increased sharply, especially for immature (II stage) females. In summary, of 151 Chinese sturgeon, whose sex and maturity stage were independently confirmed, 88.1% (n = 133), 62.9% (n = 95), and 96.7% (n = 146) were successfully sexed and staged using ultrasound, sex steroids, or both methods, respectively. The results provide reliable non-invasive techniques for determining sex and gonadal maturation of captive Chinese sturgeon. These methods can track individual gonad characteristics over multi-year reproductive cycles, which will assist captive broodstock management, artificial reproduction, and future conservation stocking. 

Hao Du, Xiaoyan Zhang, Xiaoqian Leng, Shuhuan Zhang, Jiang Luo, Zhigang Liu, Xingmei Qiao, Boyd Kynard, Qiwei Wei*.General & Comparative Endocrinology, 2017, 245: 36-43.

附件下载：/Upfile/ Gender and gonadal maturity stage identification of captive Chinese sturgeon, Acipenser sinensis, using ultrasound imagery and sex steroids 2017.pdf