Abstract: When paddlefish, Polyodon spathula, eggs were fertilized with UV irradiated (863 lW cm)2) sperm of Amur sturgeon,Acipenser schrenckii, viable gynogenetic diploid progeny with approximately 120 chromosomes occurred due to spontaneous diploidization of the maternal set of chromosomes(SDM). Distant hybridization of P. spathula eggs by A. schrenckii sperm induced inviable gynogenetic haploid progeny with approximately 60 chromosomes. When eggs gynogenetically activated by heterospecific sperm were heatshocked with 37℃ for 2 min at 18 min post-fertilization, a small proportion (7.8%) of viable gynogenetic diploid progeny appeared. The female inheritance of these gynogenetic diploid progeny was verified by chromosome analyses and microsatellite genotyping.


附件下载：Y.C.Zou, Q.W.Wei, L.B.Zeng, et al. Production of gynogenetic diploid Polyodon spathula using fertilization with irradiated sperm followed by spontaneous diploidization and distant hybridization caused by heat shock. J.Appl.Ichthyol. 2011,27: 505-509. pdf