Summary: This investigation studied the effectiveness of techniques to collect sperm from Chinese sturgeon males and developed examination methods fon the viability of sperm. Further, a series of experiments tested a variety of sperm diluents while also selecting suitable cryoprotectants and its concentration, determined optimal equilibrium time, cooling rate and freezing point. Additionally, the effects of the cryo-preservation methods on the viability of sperm were tested while also determining the fertilization success with sperm cryo-preserved with three different diluents. The results indicated that the diluent D-5 (8.85g/L NaCl; 0.20g/L and 0.40g/L NaHCO3) was the best sperm diluent, and 12% DMSO was a suitable cryo-protectant keeping 0.5-2h as the equilibrium time at 4.Combining D-5 with DMSO under the described conditions seems to be the most suitable method for sturgeon sperm cyro-proservation while employing the cooling rate at 2/min and freezing pointat -6 keeping the sperm for 10 min before placing into liquid nitrogen. Fertilization tests, D-3, D-5 and D-6, (whose effects were similar in the diluents tests), were used to compare the effects of cryopreserved sperm on fertilization success. The results indicated that the effect of D-5 in thawed sperm fertilization was the best among all three diluents having the least negative effect. The resulting in a fertilization rate was 83.8% and survival rate of larvae reached up to 68.1%. However, comparing with the control, the survival rate of D-5 was still noticeably smaller. The reason needs to further study.

附件下载：L.Liu, Q.Wei, F.Guo, et al. Cryopreservation of Chinese sturgeon (Acipenser sinensis) sperm. J.Appl.Ichthyol. 2006, 22(Suppl.1): 384-388 .pdf