This study compared the effects of 3 different types of commercial PCV2 vaccines on PCV2 virus shedding in the semen from infected boars. Twenty-five non-PCV2 viremic and seronegative boars were randomly divided into five groups: three vaccinated and challenged groups, a non-vaccinated and challenged group, and a negative control group. The number of genomic copies of PCV2 in serum and semen samples was significantly decreased in vaccinated and challenged boars compared to non-vaccinated and challenged boars from 14 to 70 days post-inoculation (dpi). The number of PCV2 genomic copy in the semen correlated with the number of PCV2b genomic copy in the blood in vaccinated and challenged boars (r2 = 0.894–0.926, P < 0.01), and non-vaccinated and challenged boars (r2 = 0.903, P < 0.01). The vaccination protocol reduced the amount of PCV2 DNA shed in the semen. However, there was a significantly different amount of PCV2 DNA shed in semen among the 3 vaccinated and challenged boar groups. Veterinary Microbiology. 2013 (164): 145-149. Hwi Won Seo, Kiwon Han, Yeonsu Oh, Ikjae Kang, Changhoon Park,Chanhee Chae *. Seoul National University, College of Veterinary Medicine, Department of Veterinary Pathology, 599 Gwanak-ro, Gwanak-gu, Seoul 151-742, Republic of Korea. swine@snu.ac.kr (C. Chae).