Abstract: The diet of Leptobotia elongata in the Yibin reach of the Yangtze River, China was investigated by stomach content analysis and by stable isotope analysis frommuscle. The results of the two methods were agreement. Both stomach contents and isotope analysis indicated that L. elongata fed in springmainly on plankton,shrimp and fish, and secondarily on benthic invertebrates and aquatic insect larvae. For the  stomach content analysis,the diet composition showed significant differences among the size classes in relative weight of prey items, with L. elongata changing feeding habits at c.110 mm standard length. The smaller individuals fed on benthic invertebrates and aquatic insect larvae, whereas individuals >109 mm fed mainly on shrimp and fish. A similar shift to piscivory at c.110mm standard length was found using the stable isotope mixing model to reveal dietary ontogeny by IsoSource software, and the trend in variation of the δ13C and δ15N was similar with increased body length, and the plankton is important prey item in all size classes. The δ13C and δ15N values in similar sized individuals showed significant seasonal differences (δ13C, ANOVA, F=76.33, p<0.001 and δ15N, ANOVA,F=144.56, p<0.001), indicating a temporal dietary and trophic level shift. L. elongata is an important commercial species, and the results of the study form part of a detailed investigation of feeding ecology of L. elongata that provides basic data for studying the food web of the upper Yangtze River.

L. Li, Q.Wei Wei*, J. MingWu, H. Zhang, Y. Liu, X. Xie. Diet of Leptobotia elongata revealed by analysis and inferred from stable isotope signatures. Environmental Biology of Fishes, 2015, 98: 1965-1978.